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人百日咳毒素酶免試劑盒,(PT)ELISA檢測試劑盒,英文名:PT ELISA Kit,英文縮寫:PT ,常見試劑盒規(guī)格:96T/48T,產(chǎn)品別名:人百日咳毒素ELISA檢測試劑盒,PT 檢測試劑盒,PT 檢測試劑盒
ELISA實驗操作較繁雜,操作不當將引起較大的誤差。人百日咳毒素酶免試劑盒,(PT)ELISA檢測試劑盒 在操作中應(yīng)注意以下5個方面。
1.隨著病情的進展或由其他因素影響,某些抗體在機體內(nèi)的含量發(fā)生大幅波動,因此有必要對標本進行預(yù)處理,例如對血清標本作適當稀釋,或離心分離血脂等。間接法測定中,標本適當稀釋還可降低非特異性反應(yīng)。
2.加樣一般使用加液器,在ELISA中一般有4次加樣:加標本、加酶結(jié)合物、加底物和加終止液。加標本時必須每份標本使用一支移液器吸嘴,以免交叉污染。加酶結(jié)合物、底物和終止液時則不需更換吸嘴。
3.在成批手工檢測中,還應(yīng)注意操作時差的影響。加樣及混勻時間的差異,使抗原抗體反應(yīng)和酶促反應(yīng)時間不*,對競爭法及定量檢測影響很大,不注意可能會導(dǎo)致錯誤結(jié)果或定量不準。
4.洗滌是ELISA操作過程中決定實驗成敗的一個關(guān)鍵步驟。目的是除去未結(jié)合的免疫反應(yīng)物,終止抗原抗體反應(yīng),除去標本中與反應(yīng)無關(guān)的成分、游離的酶標物以及反應(yīng)過程中吸附于固相載體上的非特異性物質(zhì)??捎孟窗鍣C洗板或手工洗板,前者洗滌質(zhì)量較好,各反應(yīng)孔洗滌效果均一,批內(nèi)、批間差異小,手工洗板應(yīng)注意控制各孔洗滌效果,差異不宜太大。
5.準確判定結(jié)果須使用ELISA測讀儀(酶標儀),比色法判讀可選擇單波長或雙波長,根據(jù)反應(yīng)液顏色的不同選用不同波長的濾光片,以空白孔校零測定反應(yīng)孔的吸光度值(A值)。酶標儀具有良好的重復(fù)性。
叉頭框蛋白P1(FOXP1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Forkhead Box Protein P1 (FOXP1)48T/96TY09785Homo sapiens (Human)
叉頭框蛋白O1(FOXO1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Forkhead Box Protein O1 (FOXO1)48T/96TY09786Homo sapiens (Human)
叉頭框蛋白C1(FOXC1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Forkhead Box Protein C1 (FOXC1)48T/96TY09787Homo sapiens (Human)
叉頭框蛋白O3(FOXO3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Forkhead Box Protein O3 (FOXO3)48T/96TY09788Homo sapiens (Human)
CD14分子(CD14)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Cluster Of Differentiation 14 (CD14)48T/96TY09789Rattus norvegicus (Rat)
棕櫚?;さ鞍?(MPP2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Membrane Protein, Palmitoylated 2 (MPP2)48T/96TY09790Rattus norvegicus (Rat)
乙醇脫氫酶1(ADH1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Alcohol Dehydrogenase 1 (ADH1)48T/96TY09791Rattus norvegicus (Rat)
顆粒酶K(GZMK)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Granzyme K (GZMK)48T/96TY09792Homo sapiens (Human)
血管生成素4(ANGPT4)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Angiopoietin 4 (ANGPT4)48T/96TY09793Homo sapiens (Human)
整合素αD(ITGαD)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Integrin Alpha D (ITGaD)48T/96TY09794Homo sapiens (Human)
脂多糖結(jié)合蛋白(LBP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Lipopolysaccharide Binding Protein (LBP)48T/96TY09795Rattus norvegicus (Rat)
血管生成素4(ANGPT4)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Angiopoietin 4 (ANGPT4)48T/96TY09796Rattus norvegicus (Rat)
血管生成素4(ANGPT4)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Angiopoietin 4 (ANGPT4)48T/96TY09797Mus musculus (Mouse)
心肌肌鈣蛋白I(TNNI3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Troponin I Type 3, Cardiac (TNNI3)48T/96TY09798Sus scrofa; Porcine (Pig)
堿性磷酸酶(ALP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Alkaline Phosphatase (ALP)48T/96TY09799Rattus norvegicus (Rat)
CD6分子(CD6)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Cluster Of Differentiation 6 (CD6)48T/96TY09800Canis familiaris; Canine (Dog)
堿性磷酸酶(ALP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Alkaline Phosphatase (ALP)48T/96TY09801Mus musculus (Mouse)
心鈉肽(ANP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Atrial Natriuretic Peptide (ANP)48T/96TY09802Homo sapiens (Human)
凋亡誘導(dǎo)因子(AIF)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Apoptosis Inducing Factor (AIF)48T/96TY09803Rattus norvegicus (Rat)
凋亡誘導(dǎo)因子(AIF)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Apoptosis Inducing Factor (AIF)48T/96TY09804Mus musculus (Mouse)
粘蛋白5B(MUC5B)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Mucin 5 Subtype B (MUC5B)48T/96TY09805Homo sapiens (Human)
催乳素(PRL)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Prolactin (PRL)48T/96TY09806Oryctolagus cuniculus (Rabbit)
蛋白酪氨酸磷酸酶受體N(PTPRN)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Protein Tyrosine Phosphatase Receptor Type N (PTPRN)48T/96TY09807Homo sapiens (Human)
親環(huán)素B(CYPB)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Cyclophilin B (CYPB)48T/96TY09808Homo sapiens (Human)
抑制素結(jié)合蛋白(INHBP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Inhibin Binding Protein (INHBP)48T/96TY09809Rattus norvegicus (Rat)
溶菌酶(LZM)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Lysozyme (LZM)48T/96TY09810Oryctolagus cuniculus (Rabbit)
干擾素β(IFNβ)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Interferon Beta (IFNb)48T/96TY09811Chicken (Gallus)
核糖核酸酶A3(RNASE3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Ribonuclease A3 (RNASE3)48T/96TY09812Rattus norvegicus (Rat)
血管緊張素Ⅱ受體1(AGTR1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Angiotensin II Receptor 1 (AGTR1)48T/96TY09813Homo sapiens (Human)
主要堿性蛋白(MBP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Major Basic Protein (MBP)48T/96TY09814Rattus norvegicus (Rat)
血管性血友病因子裂解蛋白酶(vWFcp)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Von Willebrand Factor Cleaving Protease (vWFCP)48T/96TY09815Rattus norvegicus (Rat)
激活素A受體Ⅰ(ACVR1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Activin A Receptor Type I (ACVR1)48T/96TY09816Rattus norvegicus (Rat)
白介素6(IL6)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Interleukin 6 (IL6)48T/96TY09817Canis familiaris; Canine (Dog)
白介素10(IL10)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Interleukin 10 (IL10)48T/96TY09818Canis familiaris; Canine (Dog)
干擾素γ(IFNγ)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Interferon Gamma (IFNg)48T/96TY09819Canis familiaris; Canine (Dog)
顆粒酶K(GZMK)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Granzyme K (GZMK)48T/96TY09820Rattus norvegicus (Rat)
Ⅲ型前膠原羧基端原肽(PⅢCP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Procollagen III C-Terminal Propeptide (PIIICP)48T/96TY09821Homo sapiens (Human)
Ⅱ型前膠原羧基端原肽(PⅡCP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Procollagen II C-Terminal Propeptide (PIICP)48T/96TY09822Homo sapiens (Human)
胰島素樣生長因子2受體(IGF2R)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Insulin Like Growth Factor 2 Receptor (IGF2R)48T/96TY09823Homo sapiens (Human)
組織內(nèi)轉(zhuǎn)化生長因子β結(jié)合蛋白2(LTBP2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Latent Transforming Growth Factor Beta Binding Protein 2 (LTBP2)48T/96TY09824Homo sapiens (Human)
絲裂原激活蛋白激酶激活蛋白激酶3(MAPKAPK3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Mitogen Activated Protein Kinase Activated Protein Kinase 3 (MAPKAPK3)48T/96TY09825Homo sapiens (Human)
骨骼肌肌動蛋白α1(ACTα1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Actin Alpha 1, Skeletal Muscle (ACTa1)48T/96TY09826Homo sapiens (Human)
葡萄糖轉(zhuǎn)運蛋白3(GLUT3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Glucose Transporter 3 (GLUT3)48T/96TY09827Homo sapiens (Human)
轉(zhuǎn)化生長因子β刺激蛋白22(TSC22)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Transforming Growth Factor Beta Stimulated Protein Clone 22 (TSC22)48T/96TY09828Homo sapiens (Human)
前列腺素D合酶(PTGDS)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Prostaglandin D2 Synthase, Hematopoietic (PTGDS)48T/96TY09829Homo sapiens (Human)
電子轉(zhuǎn)移黃素蛋白β肽(ETFβ)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Electron Transfer Flavoprotein Beta Polypeptide (ETFb)48T/96TY09830Homo sapiens (Human)
磷酸甘油酸變位酶2(PGAM2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Phosphoglycerate Mutase 2, Muscle (PGAM2)48T/96TY09831Homo sapiens (Human)
黑素瘤衍生亮氨酸拉鏈額外核因子(MLZE)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Melanoma Derived Leucine Zipper Extra Nuclear Factor (MLZE)48T/96TY09832Homo sapiens (Human)
結(jié)蛋白(Des)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Desmin (Des)48T/96TY09833Canis familiaris; Canine (Dog)
煙堿型膽堿受體β1(CHRNβ1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)
ELISA Kit for Cholinergic Receptor, Nicotinic, Beta 1 (CHRNb1)48T/96TY09834Homo sapiens (Human)
各種類型ELISA測定時, 一般需經(jīng)過以下步驟:固相包被→加待測樣品→溫育→洗滌+加酶標物→溫育→洗滌→加底物→溫育一加終止液→比色→判定結(jié)果。